The ice pack that comes with your insulated Legacy kit keeps your bottle of transport medium cool for its transit to you. You do not need to refreeze the ice pack that came with your kit.
You can keep your Legacy kit untouched and at room temperature when it arrives. We recommend using it within 7 days for optimal results.
If it's been longer than 7 days, you can refrigerate the bottle of transport fluid until you're ready to use it. Just bring that bottle to room temperature 30 minutes before you provide your sample.