It does not. The bottle of transport medium that comes with your kit can be kept at room temperature for around 7 days. You can also refrigerate the transport medium until you are ready to provide your sample. The transport medium is viable for up to one month or longer, depending on the expiry date that's printed on it.
Our partner clinic cannot accept kits that are returned over the weekend, so please make sure you provide your sample and return it on the same day, Monday-Thursday.